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104. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft 2006
Abstract
Abstract
SO.14.09 Culture of lacrimal gland acinar cells on amniotic membrane – A suitable in vitro model of lacrimal gland function? Schrader S.1, Wedel T.2, Kremling C.1, Geerling G.3
1Department of Ophthalmology, 2Department of Anatomy, Universitätsklinikum Schleswig-Holstein, Campus Lübeck; 3Department of Ophthalmology, Julius-Maximilian-University Würzburg Objective: The secretion of the lacrimal gland provides 95% of the aqueous tears, which are essential for lubrication, nutrition and protection of the ocular surface. Long-term studies of acinar lacrimal gland cells in-vitro are complicated by low proliferation rate and fast loss of cell function on plastic. Aim of this study was to analyse the effect of amniotic membrane as a carrier on the proliferation and secretory function of acinar lacrimal gland cells in a rabbit model.
Methods: Acinar lacrimal gland cells from Chinchilla Bastard- and New Zealand White rabbits of different sexes where isolated and cultured on denuded amniotic membrane in modified HSM-medium. Cells were analysed by light microscopy and transmissionelectron microscopy. Cell viability was analysed by means of the Calcein/AM-Assay and secretory response to carbachol was tested by measuring the b-Hexosaminidase activity.
Results: By day 3 single cells and small groups of cells were found on the amniotic membrane. Cells showed polarity, with a basal nucleus and large electron lucent apical granules. Between day 7 and 14 the cell groups increased in size and Acini with a central lumen could be observed. The number of electron lucent granules decreased and electron dense granules became more abundant. On day 28 cellular organisation into acini, accumulation of secretory material in the lumen and desmosome formation connecting the apical cell structures was observed. However, between day 14 and 28 the number of cytoplasmatic granules decreased and fibroblast like cells appeared. The secretory response to carbachol showed increased b-Hexosaminidase activity until day 14.
Conclusions: Acinar lacrimal gland cells can be successfully cultured on amniotic membrane up to 28 days, with a stable secretory function up to 14 days. This may be a suitable in vitro model for studying acinar cell proliferation and cell function.
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