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104. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft 2006
Abstract
Abstract
SO.14.04 Effects of contact lenses on the protein composition in tear film: A ProteinChip study Kramann C., Wiegel N., Pfeiffer N., Grus F. H.
Department of Ophthalmology, Johannes Gutenberg-University Mainz Objective: The aim of this study was to analyze the effect of hard and soft contact lenses on the protein composition of tear in contact lens wearers.
Methods: Wearers of soft contact lenses (n=13) and hard contact lenses (n=13) were recruited. A group of 13 non-contact lens wearers were also included as a control group. Tears were collected using Schirmer strips with anesthesia and frozen until use. The tears were eluted from Schirmer strips and analyzed on ProteinChips (Ciphergen, Fremont, USA) with different chromatographic surfaces (cationic and anionic exchanger, and reversed phase surface). Specific protein biomarkers were identified using ProteinChips (SELDI-TOF) with subsequent multivariate statistical analysis and artificial neural networks.
Results: The tear protein composition differed significantly from protein composition in non-contact lens wearers in both wearers of hard and soft contact lenses (P<0.01). However, the deviation of tear protein patterns were more pronounced in wearers of soft contact lenses. Nevertheless, considering typical dry-eye biomarker found in previous studies (Grus et al., IOVS 2005), e.g. the PRP4 (proline rich protein) differed in both soft and hard contact lens wearers in comparison to the non-contact lens wearers.
Conclusions: Contact lens wear alters tear protein profiles in a complex manner. This study could demonstrate that those changes can not only be found in wearers of soft contact lenses, but also in hard contact lens wearers, the tear protein composition differed significantly from the tear protein profile of non-contact lens wearers in both groups.
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